Research Summary
The Rao laboratory at Johns Hopkins studies ion transporters, including H+-ATPases, Ca2+-ATPases and Na+/H+ exchangers. 我们的研究重点是发现新的离子转运体及其在人类健康和疾病中的生理作用.We initially defined the Golgi/Secretory Pathway Ca2+, Mn2+- atp酶(SPCA)，并根据其独特的生化和系统发育特性，将其划分为一个新的家族. Our discovery of an oncogenic role for SPCA2 in breast cancer, mediated by an unconventional mechanism of signaling to Ca2+ channels, opens a new chapter in the study of this isoform. We were the first to clone and recognize the endosomal Na+(K+)/H+ 交换剂(eNHE)作为一种独立于众所周知的质膜NHE的基团. Currently, 我们结合使用酵母和神经生物学模型来了解eNHE在神经系统疾病中的作用, including autism, Alzheimer disease and glioblastoma. 最近，我们发现了一个新的后生动物家族+ antiporters that are distantly related to bacterial NhaA; our findings implicate a role for human NHA2 in sodium lithium countertransport activity linked to essential hypertension. 我们还研究了离子介导的真菌(C. albicans) cell death and target this pathway, including the V-type H+-ATPase, for the development of antimycotic drugs. 我们正在探索抗心律失常药物胺碘酮在抗真菌治疗中的应用, particularly of azole-resistant fungi.
Lab
Lab Website: Rao Laboratory
Selected Publications
Kondapalli, KC, Llongueras, JP, Capilla-Gonzalez, V, Hack, A, Smith, C, Guerrero-Cazares, H, Quinones-Hinojosa, A, and Rao, R. (2015)内体内腔质子泄漏通路驱动胶质母细胞瘤的致癌信号传导. Nature Communications 6 Article number: 6289 doi:10.1038/ncomms7289
Hack A, Schushan M, Landau M, Ben-Tal N, Rao R. "Functional evaluation of autism-associated mutations in NHE9." Nat Commun. 2013;4:2510. doi: 10.1038/ncomms3510.
Patenaude C, Zhang Y, Cormack B, Köhler J, Rao R. 白色念珠菌毒力中空泡酸化的重要作用." J Biol Chem. 2013 Sep 6;288(36):26256-64. doi: 10.1074/jbc.M113.494815. Epub 2013 Jul 24.
Cross BM, Hack A, Reinhardt TA, Rao R. “SPCA2调节Orai1的运输，并在哺乳模型中存储独立的Ca2+进入." PLoS One. 2013 Jun 28;8(6):e67348. doi: 10.1371/journal.pone.0067348. Print 2013.
Shim JS, Rao R, Beebe K, Neckers L, Han I, Nahta R, Liu JO. HIV蛋白酶抑制剂奈非那韦选择性抑制her2阳性乳腺癌细胞." J Natl Cancer Inst. 2012 Oct 17;104(20):1576-90. doi: 10.1093/jnci/djs396. Epub 2012 Oct 5.
Kondapalli KC, Kallay LM, Muszelik M, Rao R. "Unconventional chemiosmotic coupling of NHA2, a mammalian Na+/H+ antiporter, to a plasma membrane H+ gradient." J Biol Chem. 2012 Oct 19;287(43):36239-50. doi: 10.1074/jbc.M112.403550. Epub 2012 Sep 4.
Feng, M., Grice, D., Faddy, H.M., Ngyuen, N., Leitch, S., Wang, Y., Muend, S., Kenny, P.A., Sukumar, S., Roberts-Thomson, S., Monteith, G., and Rao, R. (2010)乳腺肿瘤中SPCA2对Orai1的储存无关激活. Cell 143, 84-98.
Patient Ratings & Comments
患者评分分数是通过Press Ganey对全国CG-CAHPS医疗实践患者体验调查中与医生相关问题的所有回答的平均值. 回答以1到5分进行衡量，5分是最高分. 我们还通过Press Ganey收集了CG-CAHPS医疗实践调查的评论，并将其完整地展示出来. 出于保密和患者隐私的考虑，患者的身份被取消.